Fluorescence Liftime Imaging System
Picoquant MicroTime MT200
We are very luck to work with one of the best set ups in Poland. Fluorescence Lifetime Imaging Microscopy (FLIM) is a method that is based on the measurement on the fluorescence lifetime in conjunction with a confocal microscope. FLIM measures parameters of fluorescence such as intensity, wavelength, lifetime and polarization in any given point of a microscopic sample. It can provide information about dynamic processes occurring in the sample, molecular organization of species of interest, interaction between molecules and their orientation.
The system consists of a Picoquant MicroTime 200 main module, Olympus IX71 confocal microscope; six pulse lasers, including a tunable femtosecond 690 – 1020 nm 4W laser; six picosecond diode-pumped solid-state lasers (~1 mW): 375 nm, 405 nm, 440 nm, 470 nm, 510 nm, 640 nm; two Picoquant τ-SPAD photon counting detectors (350 ps resolution); PMA hybrid detector (120 ps resolution).